1) Which super-resolution method do you advice?
2) Hardware. Name, specify and discuss all components that can be reused and those that must be purchased to fulfil the requirements of your lab. Include the pixel size of the camera into your considerations.
3) Fluorophores. Can the first fluorophore mEos2 be used for your proposed method? Suggest also an appropriate fluorophore and labelling strategy for the second colour channel and justify your choice.
4) Fluorescence filters. Specify all optical filters that are needed in the proposed microscope.
5) Resolution. How can the lateral resolution of your method be characterized? Suggest and explain a strategy to determine the resolution.
6) Correlative imaging. Your lab has also access to a scanning electron microscope (SEMI. After realization of the optical super-resolution microscope, discuss in brief strategies to perform correlative imaging.
