What is the function and mode of action of IPTG and why did you add it to the bacterial culture? Why does the pGEX vector have an ampicillin resistance gene and what protein does it code for?
Experiment I – Co-precipitation of the EGFR and PLCg SH2 domain GST-fusion proteins Insert a fully labelled image of the Experiment 1 Coomassie-stained lower half of the protein gel, indicating the migration of the molecular weight markers and identifying any bands that you can. Using the above gel image estimate the molecular weight, in kDa, […]