1. The chromophore of GFP is what gives off the bright light. In the structure shown below, circle 1 amide, 1 amine, 1 carboxylic acid, and 1 alcohol.
2. In the structure shown above, (a) how many amides are there? (b) amines? (c) carboxylic acids (d) and phenols?
3. Referring back to the Project from Module 3, what IR bands would you expect to be prominent in an IR spectrum of the chromophore shown above?
4. How are the proteins sorted on the column showed in the second video?
5. Compare and contrast the chromatography method described above for GFP to the ones you used and the one you wrote about in your project for Module 2. (100-200 words)
6. Referring back to Module 1, circle on the molecule shown below the area of the molecule that is aromatic and has conjugation. NOTE: You can either print or draw the molecule and then show your work for this question. All drawings should be signed and dated.
