What is GAL80 and why does the yeast two hybrid system require mutant strains of yeast that lack the expression of this protein?
Fields & Song, Nature, 1989 The authors state that “protein-protein interactions between two proteins have generally been studied using biochemical techniques such as cross-linking, co-immunoprecipitation and co-fractionation by chromatography.” Briefly describe one of these techniques. What does the GAL4 protein normally do in Saccharomyces cerevisiae (Brewer’s yeast)? What are the attributes of GAL4 that enable […]