How much discrepancy did you see between the two methods? If you wanted to be as accurate as possible in obtaining the MW of a protein or peptide, which method would you use and why?
Affinity chromatography protein Purification Guidelines for the Affinity Purification Lab Report 1) Estimate the molecular weights of the three prominent bands you see in your thrombin digest lane. 2) You have been provided with two results of a MALDI-TOF analysis. a) Calculate the actual molecular weight of the three bands by doing a ratio calculation, […]