Would the amount of chlorophyll be affected by resuspending the chloroplasts in water? If so, would the measured amount increase or decrease?
Resuspend the chloroplast pellet in 5.0 ml of cold suspension buffer. Use a glass stirring rod to gently disrupt the packed pellet. This is the chloroplast suspension for use in subsequent procedures. Store the suspension in the dark at 4˚C. Be sure the suspension is well mixed before removing samples from it. Determine the concentration […]