Sickle Cell Anemia: How can we alter the genome to
Present a question. What is the question that you want to answer?
Describe background of gene/condition, etc. What does your audience need to know about this? What causes it? What is the specific sequence change . How many people are affected? What are the effects? Why is this an important question to address?
Please be sure to cite sources to describe the background information, including info about the genes, the role of the genes to be targeted, overview of disease or condition resulting from mutant alleles, or an overview of the function of the genes to be
altered/fused .
3. Proposed Strategy: How will you address this? Please be specific. What will the constructs look like? Please describe your strategy for creating the sgRNA construct and the donor DNA construct, and please provide a diagram that includes the relevant sequence. In your diagram, please show: target sequence, PAM, sgRNA sequence, where mutation occurs , where cut site/s is/are. If creating a fusion
protein, please show in your diagram where your inserted gene will go. Also, please show what the sequence of your donor DNA is .
