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What was the phosphorylation status of the CTD of Pol II molecules found by the authors to be associated with promoter-proximal pausing, splicing sites and the TES? How, in your opinion, can differential phosphorylation of the CTD dictate and control the different co-transcriptional activities of transcriptional termination and splicing that take place across the gene?

With reference to the research article from Nojima et al., Cell 2015 [1], discuss the relevant data and write a series of paragraphs addressing the following points: a. Briefly describe the mNET-seq technique and explain two advantages and two disadvantages compared to i) RNA Pol II ChIP-seq method and ii) PRO-seq assays. b. Describe where […]

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