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What is GAL80 and why does the yeast two hybrid system require mutant strains of yeast that lack the expression of this protein?

Fields & Song, Nature, 1989 The authors state that “protein-protein interactions between two proteins have generally been studied using biochemical techniques such as cross-linking, co-immunoprecipitation and co-fractionation by chromatography.” Briefly describe one of these techniques. What does the GAL4 protein normally do in Saccharomyces cerevisiae (Brewer’s yeast)? What are the attributes of GAL4 that enable […]

Explain the advantages and limitations of consuming legumes as protein sources. Identify the vitamins and minerals found in legumes but not in meat. What other characteristics do legumes have that meats do not?  Are the major sources of protein in your diet plant based or animal based?  Would plant based proteins have less environmental impact?  Why or why not? 

Answer the following questions.  Remember to show all your calculations and indicate units after every number. Based on your three-day average, how many grams of protein do you consume daily? How many calories does this represent? What percent of your average calorie intake was contributed by proteins? The World Health Organization recommends between 10 to 15 […]

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